In chromatography, the resolution is a measure of the separation of two peaks of different retention time in a chromatogram. It is measured by dividing the difference in peak retention times by the average peak width.
Usp resolution calculation formula
The resolution between peaks of two components may be calculated using the following equation:
RS (resolution) = 1.18 (tR2− tR1) ÷ (Wh1+Wh2)
Where,
tR2>tR1
tR1, tR2 = retention times of the peaks
Wh1, Wh2= peak widths at half-height
In quantitative thin-layer chromatography, using densitometry, the migration distances are used instead of retention times and the resolution between peaks of two components may be calculated using the following equation:
RS = 1.18a (RF2−RF1) ÷ (Wh1+Wh2)
Where,
RF2>RF1
RF1, RF2 = retardation factors of the peaks
Wh1, Wh2 = peak widths at half-height
a = migration distance of the solvent front
Post a Comment